@article{oai:chuo-u.repo.nii.ac.jp:00001231,
 author = {甲斐, 義幸 and 小寺, 義男 and 前田, 忠計},
 journal = {中央大学理工学研究所論文集},
 month = {Mar},
 note = {application/pdf, The reactions of oxygen radicals (O−2 , 1O2, ∗OH) and other reactive oxygen species produced in vivo have been well studied relating to human aging and disease. It is supposed that one of those effects of oxidizing reagents directly alters protein structure.
In this paper, we used sodium hypochlorite (NaOCl), artificial oxidizing reagent, or its dissociated form OCl− that is strong oxidant reagent and react with many biological molecules. It resulted in chemical modification of amino-acid residues, especially Trp residue of E. Coli alkaline phosphatase (M.W. 94000) that was adopted in this experiment. And room-temperature phosphorescence (RTP) technique was also mainly used to investigate the effect of NaOCl on the structural change of protein matrix. The RTP of alkaline phosphatase (ALP) has been studied previously and is estimated that the emission is from tryptophan residue 109 buried in the protein matrix.
We observed by the RTP method that exposure of a globular protein ALP to NaOCl resulted in stepwise modification of the protein. And the further exposure of ALP to the amounts of NaOCl resulted in structural destroy of the protein.
This stepwise change of the protein structure has not been observed by fluorescence spectroscopic method used for the additional experiment.
As a conclusion, the process of oxidative modification of ALP was complicated with the structural change of protein molecule and was not shown by a simple stochastic process. We proposed here the two-step reaction model for the oxidization process by NaOCl, which include the change of the structure, surface and inner-core side-chain, of globular protein.
SDS-PAGE was also used for an observation of a total structural change of ALP in the oxidaization process., 【査読有】},
 pages = {25--33},
 title = {室温燐光法でとらえた蛋白質分子の段階的酸化と２段階モデル},
 volume = {11},
 year = {2006},
 yomi = {カイ, ヨシユキ and コデラ, ヨシオ and マエダ, タダカズ}
}