WEKO3
アイテム
Nucleotide Sequence and Characterization of the Gene Coding for a Thermostable Heme O Synthase from the Thermophilic Bacillus PS3
https://doi.org/10.24789/00001252
https://doi.org/10.24789/00001252d9cbb9da-ca84-4726-9f93-05fc405b9ad1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文を見る(PDFファイル) (660.8 kB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2012-10-01 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Nucleotide Sequence and Characterization of the Gene Coding for a Thermostable Heme O Synthase from the Thermophilic Bacillus PS3 | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24789/00001252 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
ISHIZUKA, Morio
× ISHIZUKA, Morio
× NAKAJIMA, Tsuyoshi
× CHIKIRA, Makoto
|
|||||||||||
| 著者別名 | ||||||||||||
| 識別子Scheme | WEKO | |||||||||||
| 識別子 | 23521 | |||||||||||
| 姓名 | 石塚, 盛雄 | |||||||||||
| 著者別名 | ||||||||||||
| 識別子Scheme | WEKO | |||||||||||
| 識別子 | 23374 | |||||||||||
| 姓名 | 千喜良, 誠 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The whole caaF-caaE-caaABCD gene cluster, contains two open reading frames (caaE and caaF seem to be coding for the proteins related to heme biosynthesis pathway) on the 5’-upstream of the caa3-type cytochrome c oxidase structural genes (caaA, caaB, caaC, and caaD), was isolated from a genomic DNA library of the thermophilic bacterium Bacillus PS3. The caaE gene and its flanking regions were identified. The deduced amino acid sequence of the caaE product is composed of 309 amino acid residues, and is homologous to that of the cyoE product (heme O synthase) of bo-type ubiquinol oxidase operon from Escherichia coli. The caaE is located on the 5’-upstream of the caaA (corresponds to subunit II of PS3 cytochrome c oxidase) apart from 77 base pairs, and there are a large stem-loop structure and promoter-like structures in this region. This promoter-like structure was needed for the in vivo expression of caaA from PS3 in E. coli. The caaF is located on the 5’-upstream of the caaE apart from 175 base pairs in the reverse direction. A recombinant E. coli cell harboring the caaF-caaE-caaABCD gene cluster of PS3 synthesized heme A. The product of caaE (CaaE) was expressed in E. coli, and had a thermostable heme O synthase (protoheme IX farnesyltransferase) activity in cytoplasmic membrane [Saiki, K., Mogi, T., Ishizuka, M., and Anraku, Y., FEBS Lett. 351(2), 385-388, 1994]. Furthermore, PS3 CaaE amino acid residues as candidates for taking part in prenyl binding, transfer, or structure preserving, and for participating heme B binding or structure preserving by the comparison among CaaE homologues are discussed. |
|||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | 【査読有】 | |||||||||||
| 書誌情報 |
中央大学理工学研究所論文集 巻 7, p. 11-28, 発行日 2002-03-31 |
|||||||||||
| 出版者 | ||||||||||||
| 出版者 | 中央大学理工学研究所 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1343-0068 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | この資料の著作権は、資料の著作者または学校法人中央大学に帰属します。著作権法が定める私的利用・引用を超える使用を希望される場合には、掲載誌発行部局へお問い合わせください。 | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
