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室温燐光法でとらえた蛋白質分子の段階的酸化と２段階モデル
https://doi.org/10.24789/00001221
https://doi.org/10.24789/0000122175f40735-336f-488a-9b7c-a4101da6d565
| 名前 / ファイル | ライセンス | アクション |
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本文を見る(PDFファイル) (268.8 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2012-10-01 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | 室温燐光法でとらえた蛋白質分子の段階的酸化と２段階モデル | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Room-temperature Phosphorescence observation for NaOCl Oxidation of Alkaline Phosphatase and Two-steps Oxidation Model | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 言語 | jpn | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| ID登録 | ||||||||||||||||||
| ID登録 | 10.24789/00001221 | |||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||
| 著者 |
甲斐, 義幸
× 甲斐, 義幸
× 小寺, 義男
× 前田, 忠計
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| 著者別名(英) | ||||||||||||||||||
| 識別子Scheme | WEKO | |||||||||||||||||
| 識別子 | 23677 | |||||||||||||||||
| 姓名 | KAI, Yoshiyuki | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 著者別名(英) | ||||||||||||||||||
| 識別子Scheme | WEKO | |||||||||||||||||
| 識別子 | 23680 | |||||||||||||||||
| 姓名 | KODERA, Yoshio | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 著者別名(英) | ||||||||||||||||||
| 識別子Scheme | WEKO | |||||||||||||||||
| 識別子 | 23681 | |||||||||||||||||
| 姓名 | MAEDA, Tadakazu | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | The reactions of oxygen radicals (O−2 , 1O2, ∗OH) and other reactive oxygen species produced in vivo have been well studied relating to human aging and disease. It is supposed that one of those effects of oxidizing reagents directly alters protein structure. In this paper, we used sodium hypochlorite (NaOCl), artificial oxidizing reagent, or its dissociated form OCl− that is strong oxidant reagent and react with many biological molecules. It resulted in chemical modification of amino-acid residues, especially Trp residue of E. Coli alkaline phosphatase (M.W. 94000) that was adopted in this experiment. And room-temperature phosphorescence (RTP) technique was also mainly used to investigate the effect of NaOCl on the structural change of protein matrix. The RTP of alkaline phosphatase (ALP) has been studied previously and is estimated that the emission is from tryptophan residue 109 buried in the protein matrix. We observed by the RTP method that exposure of a globular protein ALP to NaOCl resulted in stepwise modification of the protein. And the further exposure of ALP to the amounts of NaOCl resulted in structural destroy of the protein. This stepwise change of the protein structure has not been observed by fluorescence spectroscopic method used for the additional experiment. As a conclusion, the process of oxidative modification of ALP was complicated with the structural change of protein molecule and was not shown by a simple stochastic process. We proposed here the two-step reaction model for the oxidization process by NaOCl, which include the change of the structure, surface and inner-core side-chain, of globular protein. SDS-PAGE was also used for an observation of a total structural change of ALP in the oxidaization process. |
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| 内容記述 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 【査読有】 | |||||||||||||||||
| 書誌情報 |
中央大学理工学研究所論文集 巻 11, p. 25-33, 発行日 2006-03-31 |
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| 出版者 | ||||||||||||||||||
| 出版者 | 中央大学理工学研究所 | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 1343-0068 | |||||||||||||||||
| 権利 | ||||||||||||||||||
| 権利情報 | この資料の著作権は、資料の著作者または学校法人中央大学に帰属します。著作権法が定める私的利用・引用を超える使用を希望される場合には、掲載誌発行部局へお問い合わせください。 | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
